سامانه همایش های دانشگاه جیرفت

صفحه اصلی / سومین همایش بین المللی و چهارمین همایش ملی کشاورزی، محیط زیست و امنیت غذایی

Efficacy of Microencapsulated Trichoderma Biocompounds on Biocontrol of Citrus Nematode (Tylenchulus semipenetrans)

نویسندگان :

Nilofar Arab Sarbizhan¹ Hossein Alaei Shah Vali Anar² Laleh Ilkhan³ Ali Akbar Mohammadi-Meyrik⁴

1- دانشگاه ولی عصر (عج)رفسنجان 2- دانشگاه ولی عصر (عج)رفسنجان 3- مرکز تحقیقات آموزش کشاورزی و منابع طبیعی جنوب کرمان 4- دانشگاه ولی عصر (عج)رفسنجان

کلمات کلیدی :

antagonist fungi،biological control،encapsulation،Slow decline

چکیده :

The citrus nematode (Tylenchulus semipenetrans Cobb) is one of the most important root nematodes and a key factor in branch dieback in citrus orchards, leading to a gradual decline in tree health and a 10%–30% reduction in yield. Application of biological control methods as part of integrated management can be considered in order to achieve sustainable agricultural goals. Purpose: The aim of this study is to investigate the effect of microencapsulating a mixture of plant bioactive compounds and Trichoderma on the biocontrol of citrus nematodes. Encapsulating Trichoderma bioactive compounds enhances the fungus's activity by prolonging its effectiveness and improving its efficiency through controlled release. Research Methods: An in vitro test was conducted to investigate the effect of crude plant extract, Trichoderma and commercial nematicide on the mortality of second-stage juvenile larvae with six replicates. To evaluate the mortality of nematode, 100 second-stage juvenile larvae were placed in each Petri dish plates, then a specific volume of the extract with a ratio of 1:1 of the appropriate concentrations was applied to each treatment. Findings: The results showed the mortality of second-stage juvenile larvae and egg mass hatching were significantly affected by treatment type and exposure time. The highest mortality of second-stage juvenile larvae was observed after 48 hours with isolates T-79-2 and T-93-6 exhibiting mortality rates of 69.93% and 64.59% respectively, compared to the control. As a result, the treatments had a lethal effect on the mortality of second-stage juvenile larvae and inhibited egg hatching within the egg mass. Conclusion: According to the results of previous studies and their compatibility with this study, it was shown that biological compounds based on Trichoderma fungi are effective in biological control of citrus nematodes, mitigating tree wilting and deterioration, and enhancing yield per unit area. These results provide a foundation for further applied research in biological nematode control.